LifeGlobal® HTF w/HEPES & Phenol Red LGFR 培養基
原廠網站 Click here使用HEPES和酚紅的HTF。設計用於在CO2培養箱外washing接觸材料和配子。
Indications for Use
For human oocyte retrieval and washing outside of a CO2 incubator.
Storage and Shelf Life
Store at 2-8°C and protected from light. Fourteen (14) weeks from the date of manufacture.
Composition
A HEPES-buffered medium is required when gametes and embryos are handled outside of a CO2 incubator.
Sodium Chloride, Potassium Chloride, Calcium Chloride, Potassium Phosphate, Magnesium Sulfate, Sodium Bicarbonate, Glucose, Lactate Na Salt, Sodium Pyruvate, Phenol Red, HEPES, Gentamicin Sulfate* (10 µg/ml)
*from therapeutic-grade source material
QUALITY CONTROL
Physicochemical Tests
pH 7.2-7.4
Osmolality 280-292 mOsM
Biological Tests
Endotoxin (LAL) < 0.5 EU/ml
Sterility Test (bacterial and fungal screen, SAL 10–3) PASS
Biological Assays 1-cell Mouse Embryo Assay (% expanded blastocysts at 96 h of culture after 1 h exposure) > 80%