LIFEGLOBAL® GMHT HTF培養基
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高品質的受精和卵裂胚胎培養基。配方已經過10多年的驗證。
可用於第1天至第3天的受精和胚胎培養。
| Product | Order Code | Description | Volume (mL) |
|---|---|---|---|
| HTF | GMHT-100 | High quality fertilization and cleavage embryo culture media | 100 |
| HTF | GMHT-500 | High quality fertilization and cleavage embryo culture media | 500 |
Indications for Use
Culture of cleavage-stage human embryos (Day 1 to Day 3).
Storage and Shelf Life Store at 2-8°C and protected from light. Ten (10) weeks from the date of manufacture.
Composition
A bicarbonate-buffered medium replete with glucose, lactate, and pyruvate is required to support the growth and development of human embryos in vitro. Sodium Chloride, Potassium Chloride, Calcium Chloride, Potassium Phosphate, Magnesium Sulfate, Sodium Bicarbonate, Glucose, Lactate Na Salt, Sodium Pyruvate, Phenol Red, Gentamicin Sulfate* (10 µg/ml) *from therapeutic-grade source material
QUALITY CONTROL SPECIFICATIONS
|
Assay (performed for each batch) |
Specification |
|
Physicochemical Tests |
|
|
pH (with 5% CO2) |
7.2-7.4 |
|
Osmolality |
260-270 mOsM |
|
Biological Tests |
|
|
Endotoxin (LAL) |
≤ 0.5 EU/ml |
|
Sterility Test (bacterial and fungal screen, SAL 10–3) |
PASS |
|
Biological Assays |
|
|
1-cell Mouse Embryo Assay (% expanded blastocysts at 96 h of culture) |
≥ 80% |
Special Note on the CO2 Concentration in the Incubator:
In most cases, a 5-7% concentration of CO2 in the incubator will produce a pH of 7.2 to 7.4 in HTF. However, the exact concentration of CO2 required to produce the optimum pH of approximately 7.30 (7.27-7.33) depends on several factors, including the physical characteristics of incubator and the altitude. Consequently, we strongly recommend that each laboratory determine and use the concentration of CO2 that is required to produce a pH of 7.30 in HTF.
INSTRUCTIONS FOR USE
The procedures described below have been found to be effective for the culture of human embryos. Every laboratory must define and optimize its own procedures. After each time the original bottle is opened recap the bottle tightly and store at 2-8ºC, protected from light. Twenty-four (24) hours prior to the use of HTF for embryo culture, supplement the medium with either Human Serum Albumin (HSA) or LifeGlobal® Protein Supplement to achieve desired % (v/v) of protein supplementation.