global® total® w/ HEPES LGTH 培養液含蛋白質w/HEPES
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HEPES buffered
Ready-to-use 已含 5 mg/ml LifeGlobal® Protein Supplement 蛋白質
可用於人類卵母細胞和胚胎的清洗和操作、ICSI 受精、胚胎植入
|
Product |
Order Code |
Description |
Volume |
LGPS mg/ml |
|
global total w/ HEPES |
LGTH-050 |
HEPES buffered, for washing and manipulation of oocytes and embryos, including ICSI |
50 |
5 mg/mL |
Indications for Use
Human oocyte and embryo washing and manipulation, fertilization by intracytoplasmic sperm injection (ICSI), embryo transfer.
Storage and Shelf Life
Store at 2-8°C and protected from light. Ten (10) weeks from the date of manufacture.
Disposal Consideration
Treat or dispose of waste material in accordance with all local state/provincial, and national requirements. Dispose with laboratory waste.
Composition
A HEPES-buffered medium is required when oocytes and embryos are handled outside of a CO2 incubator. Sodium Chloride, Sodium Pyruvate, L-Arginine, L-Threonine, Potassium Chloride, L-Alanine, L-Cystine, L-Tryptophan, Calcium Chloride, L-Asparagine, L-Histidine, L-Tyrosine, Potassium Phosphate, L-Aspartic Acid, L-Isoleucine, L-Valine, Magnesium Sulfate, L-Glutamic Acid, L-Leucine, Glycyl-L-Glutamine, Sodium Bicarbonate, Glycine, L-Lysine, EDTA, Glucose, L-Proline, L-Methionine, Phenol Red, Sodium Lactate, L-Serine, L-Phenylalanine, HEPES, Human Serum Albumin* (4.4 mg/ml), Human α- and β-globulins* (0.6 mg/ml), Gentamicin Sulfate* (10 µg/ml) *from therapeutic-grade source material
QUALITY CONTROL SPECIFICATIONS
|
Assay (performed for each batch) |
Specification |
|
Physicochemical Tests |
|
|
pH (with 5% CO2) |
7.2-7.4 |
|
Osmolality |
260-270 mOsM |
|
Biological Tests |
|
|
Endotoxin (LAL) |
≤ 0.5 EU/ml |
|
Sterility Test (bacterial and fungal screen, SAL 10–3) |
PASS |
|
Biological Assays |
|
|
1-cell Mouse Embryo Assay (% expanded blastocysts at 96 h of culture) |
≥ 80% |
INSTRUCTIONS FOR USE
The procedures described below have been found to be effective for human oocyte and embryo washing and manipulation, ICSI or embryo transfer. Every laboratory must define and optimize its own procedures.
1. For oocyte and embryo washing and manipulation, prepare culture dishes containing 25-100 μl droplets under oil, or in larger volumes (0.5-1.0 ml) of global® total® with HEPES, according to general laboratory practice.
2. Before introducing the oocytes or embryos, warm the culture dishes for sufficient time to ensure temperature equilibration.
3. For washing, pass the oocytes or embryos through several droplets of global® total® w/ HEPES, according to general laboratory practice.
4. Before ICSI or other manipulation, wash the oocytes or embryos, according to general laboratory practice.
5. For ICSI, prepare 5-10 small droplets (5-10 ul) of global® total® w/ HEPES in the ICSI dish, next to the PVP droplets and cover them with oil, according to general laboratory practice. Transfer the oocytes to the respective droplets just before the ICSI procedure.
6. For transfer, wash the embryos according to general laboratory practice, and transfer to the uterus in 20-30 μl of global® total® w/ HEPES.
7. Immediately prior to transfer, rinse the transfer catheter with global® total® w/ HEPES.