global® LGGG 單一階段連續性培養液

Indications for Use

Culture of human embryos from zygote to blastocyst, embryo transfer.

Storage and Shelf Life

Store at 2-8°C and protected from light. Ten (10) weeks from the date of manufacture.

Composition

A bicarbonate-buffered medium replete with glucose, lactate, pyruvate and all 20 amino acids is optimal to support the growth and development of human embryos in vitro.

Sodium Chloride, Sodium Pyruvate, L-Arginine, L-Threonine, Potassium Chloride, L-Alanine, L-Cystine, L-Tryptophan, Calcium Chloride, L-Asparagine, L-Histidine, L-Tyrosine, Potassium Phosphate, L-Aspartic Acid, L-Isoleucine, L-Valine, Magnesium Sulfate, L-Glutamic Acid, L-Leucine, Glycyl-L-Glutamine, Sodium Bicarbonate, Glycine, L-Lysine, EDTA, Glucose, L-Proline, L-Methionine, Phenol Red, Sodium Lactate, L-Serine, L-Phenylalanine, Gentamicin Sulfate* (10 μg/ml)

*from therapeutic-grade source material

QUALITY CONTROL SPECIFICATIONS

Special Note on the CO2 Concentration in the Incubator: In most cases, a 5-7% concentration of CO2 in the incubator will produce a pH of 7.2 to 7.4 in global®. However, the exact concentration of CO2 required to produce the optimum pH of approximately 7.30 (7.27-7.33) depends on several factors, including the physical characteristics of incubator and the altitude. Consequently, we strongly recommend that each laboratory determine and use the concentration of CO2 that is required to produce a pH of 7.30 in global®.

INSTRUCTIONS FOR USE

The procedures described below have been found to be effective for the preparation of global® for culture of human embryos from zygote to blastocyst, and embryo transfer. Every laboratory must define and optimize its own procedures.

After each time the original bottle is opened recap the bottle tightly and store at 2-8ºC, protected from light.

Twenty-four (24) hours prior to the use of global®, supplement the medium with either Human Serum Albumin (HSA) or LifeGlobal® Protein Supplement to achieve desired % (v/v) of protein supplementation.

1. Using a sterile pipette or tip, dispense 25-100 µl droplets or in larger volumes (0.5-1.0 ml) of global® supplemented with protein. Cover droplets with appropriate oil.
2. Before introducing the embryos, place the culture dishes in the incubator for a minimum of 8 hours to ensure CO2 and temperature equilibration. Label each dish with patient information.
3. On Day 1, place the zygotes into the equilibrated global®. Culture the embryos for 48 h (Day 3, 4-8 cell stage).
4. For further culture to the blastocyst stage: either a) transfer the cleavage-stage embryos to fresh medium under fresh oil and return to the incubator or b) maintain the embryos in the same medium (See Reed et al., 2009; 2010). Note that such uninterrupted culture requires special attention to air quality.
5. For transfer on Day 3 (cleavage stage) or Day 5/6 (blastocyst stage) follow general laboratory practice, and transfer to the uterus in 20-30 µl of equilibrated global® supplemented with protein.