global® total® LGGT 單一階段連續性培養液加蛋白質
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Let the Embryos Choose! 可用於不間斷胚胎培養的培養基，支持人類胚胎從受精卵到囊胚培養和胚胎植入而設計
LGGT已含5 mg/ml LifeGlobal® Protein Supplement
有足夠的能量物質和必需胺基酸，支持胚胎生長和發育
Global 培養液的表現已透過持續15年的使用和超過500個已發表的獨立文章使用global medium得到證明
|
Product |
Order Code |
Description |
Volume |
LGPS mg/ml |
|
global total |
LGGT-030 |
Bicarbonate buffered, for D1-5 embryo culture and transfer |
30 |
5 mg/mL |
Indications for Use
Culture of human embryos from zygote to blastocyst, embryo transfer.
Storage and Shelf Life
Store at 2-8°C and protected from light. Ten (10) weeks from the date of manufacture.
Disposal Consideration
Treat or dispose of waste material in accordance with all local state/provincial, and national requirements. Dispose with laboratory waste.
Composition
A bicarbonate-buffered protein-supplemented medium replete with glucose, lactate, pyruvate and all 20 amino acids is optimal to support the growth and development of human embryos in vitro.
Sodium Chloride, Sodium Pyruvate, L-Arginine, L-Threonine, Potassium Chloride, L-Alanine, L-Cystine, L-Tryptophan, Calcium Chloride, L-Asparagine, L-Histidine, L-Tyrosine, Potassium Phosphate, L-Aspartic Acid, L-Isoleucine, L-Valine, Magnesium Sulfate, L-Glutamic Acid, L-Leucine, Glycyl-L-Glutamine, Sodium Bicarbonate, Glycine, L-Lysine, EDTA, Glucose, L-Proline, L-Methionine, Phenol Red, Sodium Lactate, L-Serine, L-Phenylalanine, Human Serum Albumin* (4.4 mg/ml), Human α- and β-globulins* (0.6 mg/ml), Gentamicin Sulfate* (10 µg/ml)
*from therapeutic-grade source material
QUALITY CONTROL SPECIFICATIONS
|
Assay (performed for each batch) |
Specification |
|
Physicochemical Tests |
|
|
pH (with 5% CO2) |
7.2-7.4 |
|
Osmolality |
260-270 mOsM |
|
Biological Tests |
|
|
Endotoxin (LAL) |
≤ 0.5 EU/ml |
|
Sterility Test (bacterial and fungal screen, SAL 10–3) |
PASS |
|
Biological Assays |
|
|
1-cell Mouse Embryo Assay (% expanded blastocysts at 96 h of culture) |
≥ 80% |
Special Note on the CO2 Concentration in the Incubator: In most cases, a 5-7% concentration of CO2 in the incubator will produce a pH of 7.2 to 7.4 in global® total®. However, the exact concentration of CO2 required to produce the optimum pH of approximately 7.30 (7.27-7.33) depends on several factors, including the physical characteristics of incubator and the altitude. Consequently, we strongly recommend that each laboratory determine and use the concentration of CO2 that is required to produce a pH of 7.30 in global® total®.
INSTRUCTIONS FOR USE
The procedures described below have been found to be effective for the culture of human embryos from zygote to blastocyst, embryo transfer and are offered only as examples. Every laboratory must define and optimize its own procedures.
- Prepare culture dishes containing 25-100 µl droplets or in larger volumes (0.5-1.0 ml) of global® total® under oil, according to general laboratory practice.
- Before introducing the embryos, place the culture dishes in the incubator for sufficient time to ensure CO2 and temperature equilibration. Depending on the exact configuration, this may take from 24-48 hours. Equilibration will require less time if the oil and medium have been preequilibrated.
- On Day 1, place the zygotes into the equilibrated global® total®. Culture the embryos for 48 h (Day 3, 4-8 cell stage).
- For further culture to the blastocyst stage:
either a) transfer the cleavage-stage embryos to fresh medium under fresh oil and return to the incubator
or b) maintain the embryos in the same medium (See Reed et al., 2009; 2010). Note that such uninterrupted culture requires special attention to air quality.
5. For transfer on Day 3 (cleavage stage) or Day 5/6 (blastocyst stage) follow general laboratory practice, and transfer to the uterus in 20-30
µl of equilibrated global® total®.
6. Immediately prior to transfer, rinse the transfer catheter with global® total®.